Thermodynamics of left-handed

نویسنده

  • H. H. Klump
چکیده

The thermodynamics of rightand left-handed helix formation by poly[d(G-C)].poly[d(G-C)] and by poly(dG-mSdC).poly(dG-msdC) were measured spectrophotometrically and calorimetrically. From the spectrophotometric measurements the thermal stabilities of the alternative helical conformations were evaluated as a function of counterion concentration. From the calorimetric measurements the enthalpies of either right-handed or left-handed helix formation were determined. The corresponding experimental AH values are -8.6 and -11.2 kcal/mol base pairs for the two conformations in poly[dG-C)].poly[d(G-C)], and -9.0 and -12.7 kcal/mol base pairs, respectively, for poly(dG-m”dC).poly(dG-m*dC).

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تاریخ انتشار 2001